In my time in the Stevens group, I have been involved in a number of collaborative projects seeking to develop cell-interfacing biomaterials and bioimaging methods. This includes:
Ultrasound-triggered Enzyme Catalysis and Enzymatic Hydrogelation
This study was led by Dr Valeria Nele during her PhD at Imperial College. We used ultrasound to controllably permeabilize liposomes, releasing calcium ions that could then activate an enzyme (transglutaminase) to catalyze the gelation of fibrinogen (Advanced Materials 2020). This is linked to my research activity in ultrasound manipulation.
Regulation of Stem Cells using Silicon Nanoneedle Arrays
This study was led by Dr Hyejeong Seong during her postdoc at Imperial College London. We showed that nondegradable nanoneedle arrays could be used for the long-term culture of stem cells, and that tip sharpness could be used to regulate stem cell alignment, nuclear impingement, and gene expression (ACS Nano 2020).
This study was led by Dr Sahana Gopal during her PhD at Imperial College London. We developed a technique for using immunogold labelling in combination with focussed ion beam scanning electron microscopy (FIB-SEM), which we used to visualize protein expression and cell ultrastructure in 3D (Advanced Materials 2019).
Casting Hydrogels with Bio-instructive Gradients
This study was led by Dr Chunching Li during his PhD at Imperial College London. We developed a new method to programme gradients of slow-releasing osteogenic morphogens into cellularized hydrogels, which generated integrated osteochondral tissue constructs (Advanced Materials 2019). This is linked to my research activity in tissue engineering.
If you are interested in these topics, you can find more information in these recent reviews (Trends in Biotechnology 2020, Advanced Functional Materials 2020, and Advanced Functional Materials 2020).